RCFF Analysis List

  • Fiber fraction:
    (NDF- ADF- ADL- Hemicellulose – Cellulose – Lignin)

  • Dietary fiber
    Total Dietary Fiber (TDF), Soluble Dietary Fiber (SDF), Insoluble Dietary Fiber (IDF), Total Digestive Nutrients (TDN).

  • Metalic elements, macro and micro nutrientsu:
    Sodium, potassium, calcium, phosphorus, magnesium, manganese, copper, iron, boron, molybdenum,  ..etc.
    chlorine, flourine, bromine, sulfur, nitrate nitrogen, ammoniacal nitrogen, etc.
    arsenic, mercury, lead, nickel, cadmium, …etc

  • Organic carbon and total organic matter

  • Total ash, Water soluble, Acid Soluble Ash as well as insoluble acid/water ash.

  • Different cations: 
    Ammonium, Calcium, Magnesium, ….etc.

  • Different anions:
    Fluoride, chloride, nitrate, nitrite, sulfate, phosphate, acetate, propionate, maliate, maleate, …etc

  • Phyto Hormones:
    Gibberellic acid, Hydrogen cyanamide, Naphthelenea cetamide. naphthoxy acetic acid. Abscisic acid.   
    Salicylic acid. Auxin (Indoleacetic acid). Jasmonic acid. P-nitro phenol. Auxin (Indolebutyric acid).  
    P-nitro guaiacol. CPPU {N-(2-Chloro-4-Pyridyl)-N'-PhenylUrea} Chlorpropham. 
    Cytokinene (6-Benzyl adenine). O-nitro phenol. Mepquate chloride, Uniconazole Chloromequate chloride. 
    Cytokinene (Kinetin).   Triacontanol. Ethephon Pyraflufen. Naphthelene acetic acid.  Paclobutrazol. Daminozide.

  • Organic compounds: 
    Allicin. Butanol. Acrylamide. Benzen. Acetone. Isoprene. Carnitine.  Formaldehyde.

  • Essential oils: 
    Thymol. Carvacrol. Menthol. Cinnamaldehyde. Cineol.

  • Sugar alcohols: 
    Sorbitol. Xylitol. Mannitol

  • Amino Acids: 
    Total amino acids, L. lysine- Hcl, D.L  Methionine …etc , Methionine hydroxy analogue

  • Fatty acids:
    Fatty acids composition,  Peroxide Value (PV) iodine method,  P-Ansidine, Thiobarbituric Acid  For Oil Samples  (TBA), 
    Free Fatty Acids (FFA) , Iodine Value  (IV), Impurities.

  • Enzymes:  
    Phytase- Protease – Cellulase – Xylanase – beta-Glucanase – alpha and beta-Amylase – Mannanase, pectinase. ext......

  • Protein tests: Protein (Kjeldahal and Dumas)Nitrogen in FertilizerN.P.N

  • Biology Experimental: 
    Ad – libtum experiment . Biological value. True digestibility. Net protein utilization.

  • Antioxidant activity assays:
    Total Antioxidant capacity, Total PhenolsTotal Flavonoids, Ferric Reducing Power activity, 
    Cupric Reducing power activity, DPPH scavenging activity

  • Antinutrients: 
    Phytic acid, Trypsin inhibitor, alkaloids, Total oxalates, Tannins, Saponin.

  • Grain inspection:
    Yellow corn, Soybeans, Barley, Sorghum, Wheat.
    Moisture, Test Weight,  Broken Kernels &Foreign Material, Shrunkel Kernels, Splits Kernels, Heat Damage, Total Damage, Odor, Insect

  • Ash, Moisture; Silica,

  • Mycotoxins
    Aflatoxin, Ochratoxin, Zeralinon, DON, Fuminsine, Aflatoxin M1

  • Biological fluid analysis:
    Cholestrol , triglycerides, HDL cholesterol LDL cholesterol, Glucose, creatinine, 
    Got, GPT, Uric acid, urea, Alkaline phosphatase, acid phosphatase, 
    serum Alpha hetoprotein, Biliru bin (Total & Direct), Amylase, Magnesium, Iron , 
    phosphorus, Ionized calcium.
    Anti –Oxidant tests: SOD, TAC, NO, Vitamin C, GSH, GR, GPX, GST, MDA-TBars,CAT, H2O2.

  • Gross Energy

  • Microscopic Analysis: 
    Detection of animal source and estimation of its percent in compound feeding stuffs.
    Detection of plant source and estimation of its percent (cereals , legumes and meals).

  •  Vitamins: 
    Vitamin A, Vitamin E, Vitamin D, VitaminB1, VitaminB2, VitaminB6, VitaminB12, 
    VitaminB5, VitaminB3, Vitamin C, Vitamin B9, Biotin

  • Carbohydrates: 
    Mannan  ¡Glucan and Fructioligo SacharidesBetaineInositol

  • Melamine and Cyanouric acid

  • Acrylamide

  • Anticoccidials



  • Dyes:

  • Food contaminants:
    HMF, Malachite green, Melamine& cyanuric acid   

  • Sweeteners:
    Sodium cyclamate  

  • Microbiology analysis:
    Detection of salmonella in food and feed.
    Antibacterial activity test

  • Wheat and flour analysis: 
    Falling number, Starch damage (Ucd –AACC),  Ash Sediment of protein (ml), Moisture content
    Rheological   Analysis:   Tenacity (P), Extensibility (L)  , Baking strength (W), Configuration ratio (P/L)
    Water absorption, Tenacity, Elongation,   Extensibility,  Baking strength Configuration ratio, 
    Elasticity index,  Adapted hydration-

  • Gluten: 

  • (wet –Dry –Index-water binding in wet gluten), Rate of extraction (%), Color of flour·  Antibiotic sensitivity test

  • Antibiotic residues in food and feed

  • Antibacterial growth promoters in premixes

  • Bacteriostatic and bactericidal effect of antiseptics.

  • Microbial and fungal count.

  • Pathogenic bacteria identification.

  • Indication of indicator bacteria
    Enterococci, total coliform, faecal coliform, total enterobacteriaceae,  staphylococcus count. 
    (which indicates bad handling)

  • Probiotic bacteria count and identification.

  • Bacterial categorization: 
    Aerobic bacteria, Anaerobic bacteria, sporeforming aerobes and anaerobes, 
    thermophilic bacteria, psychrophilic bacteria, thermoduric bacteria. 

  • Identification of food spoilage bacteria.
    Lipolytic bacteria, proteolytic bacteria, sulphite reducing aerobes and anaerobes, Clostridium count.

  • Identification of Food Poisoning Bacteria:-
    Bacillus cereus, brucella species, campylobacter sp., Clostridium perfringins, Clostridium botulinum, E.coli 0157:H7, Kliebseilla species,Llisteria monocytogenes, Pseudomonas Gerogenoso, Salmonella species, Shigella species, Staphylococcus aureus, Vibrio species, Yesinia enterocolitica .
      Anti-moulds Activity of premixes.

  • Minimum inhibitory concentration of active ingredient or any chemical compound

  • Yeast and Mould enumeration and identification

  • All food, feed, water and airborne fungi
    Aspergillus species, Fusarium sp., Penecillium sp., Trichoderma spp., Eurotium spp., 


  • Water analysis 
    Hardness, Carbonate & bicarbonate, Acidity and alkalinity,    
    Sulfate ,Chloride, Flouride, Chlorine, Chemical Oxygen DemandÇ , 
    Biological  Oxygen Demand, Disolved oxygen, Total Solids, TDS, , 
    Salinity and Conductivity Sp. gravity , Nitrate and Nitrite 

  • In-vitro determination by Ankom method:
     Total dry matter digestibility, indigestible fiber components, organic matter digested

  • In-vitro determination by van soest method:
    Cell- digestibility ,  NDF- Digestibility,  Hem-cell-digestibility, True digestion for digestibility dry matter, Total dry matter digestibility.

  • In-vitro determination by gas test production:
    True Dry Matter Digestibility (Tru. Dig.), Organic Matter Digestibility (OMD),
    Dry Matter Digestibility (DMD), Dry Matter Intake (DMI), Short Chain Fatty Acid(SCF), Metabolic Energy (MENet Energy Lactation (NEL), Microbial mass (Mm), Apparent Dry Matter Digestibility (App. Dig.), Gas profile test (G.P.T).  

  • Determination of parameters rumen liquor:
    Determination PH meter, Determination TVFA,s, Determination Ammonia nitrogen




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