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Equipments of Nano-Technology Lab

 

This laboratory has been established supported by the latest equipments in the field
of nanotechnology, to performe scientific research, experiments
and Nano applications, following is some of these devices

 

Cell Culture& Confocal laser Scanning Microscopy (CLSM) UNIT

Laser scanning microscope is intended for high resolution imaging of biological or material samples, whereby in contrast to wide field microscopy the specimen is illuminated in raster-fashion with a focused laser beam and the optical arrangement prevents light from out-of-focus regions of the specimen contributing to image formation.
Regions and positions: it makes scan to a specific positions which could differ in the X, Y and Z by using the motorized stage.
incubator integrated with CLSM system allows imaging to live cells for a long period of time.
 

Time series: it takes series of images of the specimen in different times sequences.
Tile scan: it makes scan to a specific area on the slide in different x and y direction by using the motorized stage.
Bleaching:To perform bleaching experiments and study dynamics of particles in cell.

 

CLSM is able to make a lot of different imaging techniques as :
Z stack: which make 3D image of the specimen by taking series of images in different Z levels

 
It has scanner head with 3 channels to detect different detector signals.

CLSM is modified light microscope which differ in:
It has laser as light source.
It has pinhole hat prevent any transmitted light except that come from the focal plane which make higher resolution power.

ELECTRON MICROSCOPE UNIT

Magnification up to 1.013 Million.
- camera magnification up to 10 Mx.  - Ultra high resolution: point-point 1 nm.   
- Lattice resolution 0.144 nm . - Electron beam diffraction.  - High brightness electron source LaB6.
- Digital image recording. - Size measurement over the Digital image.  - Sample preparation and a handling.
Additional specification:-
Specimen holder is double tilt  ( α & β ) direction, which give the ability to be sure that
 the particle is two dimension or three dimension.

 

EDX  (Electron Dispersive X-rays). 
 

 We use this unit to make semi quantitative analysis for the sample and get the chart which presents the percentage of the material in this sample

 

 

 

 

Dark Field ( DF). Illustration :-
 

We use DF techniqe as shown in the last two images, As we chose only one ring from the diffraction pattern to image the atom that give this ring. As shown in the dark field image. The glowing particle in the DF image indicate to the atoms which give this ring .
 

 

 

       

STEM (Scanning Transmission Electron Microscope).

We use this unit to make Scanning over the sample surface and make EDX point analysis while imaging with STEM., Normally we use point analysis to differentiate between different particles in STEM image , which is helpful in investigation .

 

 

 

 

Spectral Characterization

- Wavelength rang 175 – 3300 and accuracy +/- 0.1.
- High performance R928 photomultiplier tube cooled PbS photocell.
- Double out-of-phase littrow monochromator providing excellent resolution.
- Photometric range is 8 Abs with accuracy +/-0.0003.
 

Zetasizer Nano

-Zetasizer Nano provides the ability to measure particle size, zeta potential and molecular weight.
-Laser Type: HeNe gas laser.
-Laser max. output power: 4mW.
-Zetasizer Nano apply Temperature to the sample ranged from 0°C up to 90°C.


 

Fluorescence Spectrophotometer

 Intense Xenon flash lamp, coupled with optimized grating blaze angles and coatings, this gives excellent sensitivity.
Schwarzchild collections optics capture a large portion of the light from the powerful Xenon flash lamp and direct it through the sample.
Red-sensitive Photomultiplier tube detectors extend sensitivity up to 900 nm without sacrificing UV performance.
Measure and heat multiple samples
Simultaneously.
Excellent temperature control.
Stirring for the sample in the cuvette is smooth and easy to control.